This vignette will show basic functions provided by viewmaster
#load greenleaf MPAL dataset into monocle3
mpal<-readRDS(file ="/Volumes/fh/fast/furlan_s/grp/data/ddata/BM_data/healthy_bone_marrow_greenleaf.rds")
plot_cells(mpal, color_cells_by = "BioClassification", label_cell_groups = F)+scale_color_manual(values = sfc(n = 26))
#load hca bone marrow seurat object, convert to monocle using
viewmaster’s helpful function
hca<-readRDS(file = "/Volumes/fh/fast/furlan_s/grp/data/ddata/BM_data/palantir_bm_r1.rds")
hca<-seurat_to_monocle3(hca, seu_rd = "tsne")
plot_cells(hca , color_cells_by = "cell_annotation", label_cell_groups = F)+scale_color_manual(values = sfc(10, scramble = F))
#Detect common variant genes between two cell data sets
vg<-common_variant_genes(mpal, hca, top_n = 5000)